| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Payne, Jarrett M (12)
|
OHSPY |
5:09.20 |
5:06.20 |
-3.00 |
| 2 |
Gao, William (12)
|
AMPEAQ |
5:37.81 |
5:24.73 |
-13.08 |
| 3 |
Cucu, Alexandru (12)
|
LEGLSS |
5:36.03 |
5:26.57 |
-9.46 |
| 4 |
Toothman, Joshua R (12)
|
LECSI |
5:34.04 |
5:38.35 |
4.31 |
| 5 |
Pesek, Alexander E (12)
|
LEUSC |
6:02.20 |
5:43.66 |
-18.54 |
| 6 |
MacPhail, Leland S (12)
|
LEGLSS |
5:43.69 |
5:46.17 |
2.48 |
| 7 |
Powell, Trenten J (12)
|
AMBPR |
5:36.43 |
5:49.28 |
12.85 |
| 8 |
Sutterlin, Zachary A (12)
|
AMHD |
6:04.98 |
5:50.99 |
-13.99 |
| 9 |
Wilderer, Dominik F (11)
|
AMANSC |
5:30.09 |
5:51.75 |
21.66 |
| 10 |
Wooley, Preston S (12)
|
LELESD |
6:09.48 |
5:52.71 |
-16.77 |
| 11 |
Tucker, Justin K (11)
|
AMCSC |
5:50.52 |
5:53.66 |
3.14 |
| 12 |
Hartman, Luke P (12)
|
AMUPEA |
5:59.56 |
5:54.56 |
-5.00 |
| 13 |
Thomas, Holden D (12)
|
AMFAST |
6:17.98 |
5:57.79 |
-20.19 |
| 14 |
Frank, Ryan M (12)
|
AMMLAC |
6:05.22 |
6:04.64 |
-0.58 |
| 15 |
McGrail, John L (12)
|
AMPEAQ |
6:19.56 |
6:08.53 |
-11.03 |
| 16 |
Grimenstein, Joshua A (11)
|
AMPEAQ |
6:25.71 |
6:10.04 |
-15.67 |
| 17 |
Beaver, Noah K (12)
|
LEUSTR |
6:16.25 |
6:13.25 |
-3.00 |
| 18 |
Pesek, Owen Z (11)
|
LEUSC |
6:12.83 |
6:18.69 |
5.86 |