| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Connelly, Reagan E (12)
|
AMPRA |
5:59.60 |
5:43.94 |
-15.66 |
| 2 |
Shipe, Dylayn J (12)
|
AMUN-R |
5:50.68 |
5:54.68 |
4.00 |
| 3 |
Schomaker, Anna J (12)
|
AMPRA |
6:07.47 |
6:10.33 |
2.86 |
| 4 |
Shafer, Riley A (11)
|
AMPRA |
6:19.03 |
6:23.83 |
4.80 |
| 5 |
Edner, Callahan E (12)
|
AMBPR |
6:38.73 |
6:24.04 |
-14.69 |
| 6 |
Pietropaolo, Sophia G (12)
|
AMPRA |
6:29.33 |
6:27.87 |
-1.46 |
| 7 |
Bissell, Amelia R (11)
|
AMUN-R |
6:43.01 |
6:28.70 |
-14.31 |
| 8 |
Poe, Abby S (11)
|
AMPEAQ |
6:42.12 |
6:31.58 |
-10.54 |
| 9 |
Melocchi, Grace R (12)
|
AMSASC |
7:08.42 |
6:36.01 |
-32.41 |
| 10 |
Czajkowski, Anna E (12)
|
AMNAC |
6:36.19 |
6:37.50 |
1.31 |
| 11 |
Schomaker, Allison H (11)
|
AMPRA |
7:08.05 |
6:37.76 |
-30.29 |
| 12 |
Stichler, Abigail O (12)
|
AMSASC |
7:04.80 |
6:44.65 |
-20.15 |
| 13 |
Rupert, Brooke T (11)
|
AMPRA |
7:08.67 |
6:46.06 |
-22.61 |
| 14 |
Dudkowski, Abigail E (12)
|
AMPRA |
7:01.44 |
6:46.74 |
-14.70 |
| 15 |
Hwang, Moonyoung (12)
|
AMKW |
6:49.07 |
6:56.23 |
7.16 |
| 16 |
Zatezalo, Grace S (12)
|
AMPRA |
7:08.67 |
7:09.79 |
1.12 |