| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Rupert, Rachel L (11)
|
AMPRA |
49.71 |
41.84 |
-7.87 |
| 2 |
Pomirleanu, Anamaria (11)
|
AMPRA |
42.67 |
42.83 |
0.16 |
| 3 |
Kabala, Ryleigh D (11)
|
AMPRA |
47.34 |
43.12 |
-4.22 |
| 4 |
Alpert, Leah A (11)
|
AMJCCS |
42.02 |
43.89 |
1.87 |
| 5 |
Lantzman, Anabel N (11)
|
AMJCCS |
46.95 |
45.80 |
-1.15 |
| 6 |
Davis, Nora K (11)
|
AMNAC |
47.28 |
46.42 |
-0.86 |
| 7 |
Wolfson, Brooklyne R (11)
|
AMWAAC |
49.07 |
46.83 |
-2.24 |
| 8 |
Kirkpatrick, Maura E (11)
|
AMPRA |
50.06 |
47.91 |
-2.15 |
| 9 |
Murphy, Madelynn G (11)
|
AMBWA |
55.93 |
48.64 |
-7.29 |
| 10 |
Merklin, Autumn M (11)
|
AMWAAC |
50.40 |
49.63 |
-0.77 |
| 11 |
Rice, Jillian G (11)
|
AMPRA |
58.43 |
55.09 |
-3.34 |
| 12 |
Camp, Chylar L (11)
|
AMPRA |
56.18 |
58.96 |
2.78 |
|
Shields, Madison L (11)
|
AMHAAC |
1:05.39 |
DQ |
- |
|
Piatek, Emma G (11)
|
AMPTSC |
45.91 |
NS |
- |
|
Field, Lucy J (11)
|
AMJCCS |
1:23.63 |
NS |
- |
|
King, Sophia A (11)
|
AMPRA |
48.86 |
NS |
- |
|
Kloc, Aleah (11)
|
AMCSC |
46.04 |
NS |
- |
|
Leonard, Alaina L (11)
|
AMWAAC |
53.76 |
NS |
- |