| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Schomaker, Allison H (13)
|
AMPRA |
32.52 |
28.78 |
-3.74 |
| 2 |
Shafer, Riley A (13)
|
AMPRA |
30.15 |
28.96 |
-1.19 |
| 3 |
Freberg, Kylie (13)
|
AMHAAC |
33.64 |
30.76 |
-2.88 |
| 4 |
Augustynowicz, Macie A (13)
|
AMPTSC |
33.52 |
31.34 |
-2.18 |
| 5 |
Magistro, Sophia R (13)
|
AMHAAC |
36.31 |
33.21 |
-3.10 |
| 6 |
Driscoll, Emma L (13)
|
AMHAAC |
|
33.33 |
- |
| 7 |
Rupert, Brooke T (13)
|
AMPRA |
40.89 |
33.34 |
-7.55 |
| 8 |
McChesney, Megan E (13)
|
AMPTSC |
42.50 |
34.04 |
-8.46 |
| 9 |
Heller-Matza, Lilja S (13)
|
AMJCCS |
51.56 |
37.94 |
-13.62 |
| 10 |
Alvarez, Sara L (13)
|
AMPRA |
44.37 |
39.82 |
-4.55 |
| 11 |
Eichelberger, Isabella K (13)
|
AMNAC |
39.52 |
40.02 |
0.50 |
| 12 |
Quemado, Dalisay B (13)
|
AMPRA |
43.46 |
42.21 |
-1.25 |
| 13 |
Konggaard, Annemarie (13)
|
AMPTSC |
58.21 |
52.65 |
-5.56 |
|
Khalid, Layla A (13)
|
AMPRA |
56.65 |
DQ |
- |