| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Van Woert, Keira E (11)
|
ADGUY |
34.52 |
32.95 |
-1.57 |
| 2 |
Unger-O'Shea, Nevaeh R (11)
|
ADGBY |
34.69 |
33.72 |
-0.97 |
| 3 |
Huckans, Harper L (11)
|
ADGLOY |
35.23 |
34.28 |
-0.95 |
| 4 |
Pezzuto, Ava R (11)
|
ADDACC |
34.92 |
34.43 |
-0.49 |
| 5 |
Coker, June M (12)
|
ADSARY |
37.34 |
34.65 |
-2.69 |
| 6 |
Wilson, Abigail M (11)
|
ADSARY |
35.72 |
35.42 |
-0.30 |
| 7 |
Cogan, Shannon L (11)
|
ADSARY |
36.87 |
36.49 |
-0.38 |
| 8 |
Nacheman, Ella A (11)
|
ADSARY |
35.62 |
36.56 |
0.94 |
| 9 |
Schoonmaker, Katherine E (11)
|
ADGBY |
36.81 |
38.22 |
1.41 |
| 10 |
Van Loan, Kathryn M (11)
|
ADKING |
36.51 |
38.45 |
1.94 |
| 11 |
Kowalski, Isabella A (11)
|
ADGLEN |
39.62 |
38.66 |
-0.96 |
| 12 |
Warden, Ella S (11)
|
ADSARY |
39.45 |
38.98 |
-0.47 |
| 13 |
Fanning, Samantha (11)
|
ADGUY |
36.97 |
39.06 |
2.09 |
| 14 |
Mantoo, Angelina (11)
|
ADGUY |
36.22 |
39.38 |
3.16 |
| 15 |
Chatterton, Grace (11)
|
ADDACC |
40.07 |
39.58 |
-0.49 |
| 16 |
Janicke, Rose B (11)
|
ADSARY |
40.26 |
40.56 |
0.30 |
| 17 |
McArdle, Grace B (11)
|
ADKING |
40.84 |
41.85 |
1.01 |
| 18 |
Niles, Aelan M (11)
|
ADKING |
40.97 |
41.94 |
0.97 |
| 19 |
Krawczeski, Jenna (11)
|
ADGLOY |
41.91 |
42.90 |
0.99 |
| 20 |
Moffitt, Sydney (12)
|
ADGUY |
43.76 |
43.41 |
-0.35 |
| 21 |
Brate, Zoe A (12)
|
ADGLOY |
36.62 |
45.35 |
8.73 |
|
Besha, Isla T (11)
|
ADGUY |
38.46 |
NS |
- |