| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Humby, Jessica L (13)
|
NIHAMB |
2:50.35 |
2:50.82 |
0.47 |
| 2 |
Cadle, Matthew D (15)
|
NIPVSC |
2:58.24 |
2:51.44 |
-6.80 |
| 3 |
Vallely, Kathryn C (14)
|
NIEAST |
2:53.55 |
2:54.62 |
1.07 |
| 4 |
May, Julia A (15)
|
NIEAST |
3:00.84 |
3:00.62 |
-0.22 |
| 5 |
Kwitek, Grace M (12)
|
NIEAST |
3:05.39 |
3:06.92 |
1.53 |
| 6 |
Oldman, Victoria E (14)
|
NIGISC |
3:28.51 |
3:07.49 |
-21.02 |
| 7 |
Kowell-Ure, Phoenix W (12)
|
NIEAST |
|
3:19.90 |
- |
| 8 |
Cadle, Mattison I (12)
|
NIPVSC |
|
3:20.29 |
- |
| 9 |
Lanthier, Alaina B (16)
|
NITTAC |
3:17.61 |
3:26.14 |
8.53 |
| 10 |
Hodge, Emily E (14)
|
NIHAMB |
|
3:32.20 |
- |
| 11 |
Garland, Piper E (9)
|
NIHAMB |
|
3:57.87 |
- |
|
Sarigiannis, Eliza H (14)
|
NIGISC |
|
NS |
- |