| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Misener, Leah J (9)
|
NCLOY |
51.13 |
48.11 |
-3.02 |
| 2 |
McDonough, Addyson R (10)
|
NCLOY |
54.92 |
48.46 |
-6.46 |
| 3 |
Bolyard, Macy J (10)
|
NCGCY |
|
48.47 |
- |
| 4 |
Bellissimo, Sophia G (10)
|
NCLOY |
51.62 |
48.87 |
-2.75 |
| 5 |
Nannucci, Bianca (9)
|
NCGCY |
1:02.45 |
52.86 |
-9.59 |
| 6 |
Claborn, Alyssa A (10)
|
NCLOY |
1:10.48 |
53.52 |
-16.96 |
| 7 |
Filiault, Julia K (10)
|
NCLOY |
58.41 |
55.52 |
-2.89 |
| 8 |
Okoniewski, Kayla M (10)
|
NCLOY |
|
56.15 |
- |
| 10 |
Dingman, Kasey S (9)
|
NCGCY |
|
57.87 |
- |
| 15 |
Johnson, Emory R (9)
|
NCFAST |
1:02.74 |
1:02.56 |
-0.18 |
| 16 |
Grubb, Emma M (9)
|
NCGCY |
1:35.78 |
1:18.90 |
-16.88 |
|
Bird, Alexa E (10)
|
NCGCY |
1:03.33 |
DQ |
- |
|
Hua, Claire S (10)
|
NCGCY |
1:14.05 |
NS |
- |
|
Kornatz, Maeve D (9)
|
NCGCY |
1:12.76 |
NS |
- |