| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Guthrie, Reade A (10)
|
NCSGSA |
32.10 |
33.50 |
1.40 |
| 2 |
Duggins, Grant A (9)
|
NCGCY |
35.16 |
35.53 |
0.37 |
| 3 |
Duggins, Adam M (10)
|
NCGCY |
34.81 |
36.01 |
1.20 |
| 4 |
Wehe, Isaiah J (10)
|
NCGCY |
|
36.11 |
- |
| 5 |
Wulber, Fletcher D (10)
|
NCNCAC |
38.63 |
39.15 |
0.52 |
| 6 |
Zhang, Wuche (10)
|
NCUN |
42.51 |
39.58 |
-2.93 |
| 7 |
Allen, Benjamin O (10)
|
NCLOY |
|
40.78 |
- |
| 8 |
Halpern, Maximillian N (10)
|
NCNCAC |
40.06 |
41.69 |
1.63 |
| 9 |
Parker, Grant E (10)
|
NCLOY |
41.56 |
42.44 |
0.88 |
| 10 |
Wolk, Max A (9)
|
NCNCAC |
43.15 |
44.18 |
1.03 |
| 11 |
King, Logan S (9)
|
NCLOY |
|
44.20 |
- |
| 12 |
Mitler, Charles G (9)
|
NCNCAC |
54.88 |
44.54 |
-10.34 |
| 13 |
Boehlke, Noah G (9)
|
NCNCAC |
42.35 |
45.05 |
2.70 |
| 14 |
Hawkins III, Rodney D (10)
|
NCRACY |
42.91 |
45.41 |
2.50 |
| 15 |
He, Bryan T (9)
|
NCGCY |
|
45.46 |
- |
| 16 |
Murray, Cody J (10)
|
NCLOY |
50.36 |
46.53 |
-3.83 |
| 17 |
Podgoreanu, Joseph O (10)
|
NCNCAC |
46.07 |
46.60 |
0.53 |
| 18 |
Maiorana, Aaron T (9)
|
NCNCAC |
47.24 |
47.97 |
0.73 |
| 19 |
Meck, Wyatt D (9)
|
NCSGSA |
47.56 |
48.88 |
1.32 |
| 20 |
Coltrane, Chasen A (10)
|
NCSGSA |
46.37 |
49.49 |
3.12 |
| 21 |
Thompson, Charles M (9)
|
NCSGSA |
1:06.11 |
49.98 |
-16.13 |
| 22 |
Gatling, Carl F (9)
|
NCGCY |
|
51.59 |
- |
| 23 |
Samtani, Sai J (10)
|
NCGCY |
56.08 |
52.37 |
-3.71 |
| 24 |
Harrison, Parker C (10)
|
NCNCAC |
50.99 |
53.09 |
2.10 |
| 25 |
Eggleston, Cannon S (9)
|
NCSGSA |
|
54.76 |
- |
| 26 |
Jia, George (9)
|
NCGCY |
|
1:01.28 |
- |
| 27 |
Xie, Edward C (9)
|
NCGCY |
|
1:03.06 |
- |
|
Helms, William V (9)
|
NCWA |
58.37 |
DQ |
- |
|
Cooper, Henry D (10)
|
NCGCY |
|
DQ |
- |
|
Zhang, Ximin S (9)
|
NCGCY |
|
DQ |
- |