| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Austin, Thaddeus E (16)
|
NCEAC |
50.36 |
52.54 |
2.18 |
| 2 |
Burton, Bence W (16)
|
NCEAC |
52.28 |
53.52 |
1.24 |
| 3 |
Burton, Tyler A (15)
|
NCEAC |
54.49 |
57.76 |
3.27 |
| 4 |
Hall, Judah E (16)
|
NCUN-1 |
58.68 |
58.47 |
-0.21 |
| 5 |
Dickenson, Trey D (15)
|
NCEAC |
59.06 |
58.83 |
-0.23 |
| 6 |
Johnson, Joshua C (17)
|
NCNEAL |
58.98 |
1:00.13 |
1.15 |
| 7 |
Abernathy, Harris S (15)
|
NCEAC |
55.99 |
1:00.47 |
4.48 |
| 8 |
Mahler, Max H (17)
|
NCEAC |
59.85 |
1:00.84 |
0.99 |
| 9 |
Smits, Cooper J (16)
|
NCEAC |
1:01.30 |
1:02.65 |
1.35 |
| 10 |
Williams, Hudson O (15)
|
NCEAC |
1:03.35 |
1:04.04 |
0.69 |
| 11 |
Powell, David A (16)
|
NCEAC |
1:01.48 |
1:04.05 |
2.57 |
| 12 |
Sidden, Wyatt R (15)
|
NCEAC |
1:09.50 |
1:04.44 |
-5.06 |
| 13 |
Schambra Stevens, Zack A (15)
|
NCEAC |
1:06.97 |
1:04.75 |
-2.22 |
| 14 |
Hartsough, Jackson A (16)
|
NCEAC |
1:02.85 |
1:04.78 |
1.93 |
| 15 |
Pugh, Keon M (15)
|
NCNEAL |
1:02.01 |
1:05.42 |
3.41 |
| 16 |
Raney, Grayden J (16)
|
NCEAC |
1:12.00 |
1:08.86 |
-3.14 |
| 17 |
Blue, Klayton L (16)
|
NCNEAL |
1:12.28 |
1:08.90 |
-3.38 |
| 18 |
Cox, Cullen B (15)
|
NCNEAL |
1:15.77 |
1:09.18 |
-6.59 |
| 19 |
Urtis, Salih (15)
|
NCEAC |
1:11.41 |
1:09.36 |
-2.05 |
| 20 |
O'Donnell, Colin J (15)
|
NCEAC |
1:25.99 |
1:11.24 |
-14.75 |
| 21 |
Martin, Cody S (15)
|
NCPAST |
1:29.01 |
1:14.14 |
-14.87 |
| 22 |
Rasmussen, Bridger E (17)
|
NCPAST |
1:47.45 |
1:14.63 |
-32.82 |
| 23 |
Gualteros, Dominic X (15)
|
NCNEAL |
|
1:16.81 |
- |