| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Ross, Sadie B (10)
|
NCGCY |
29.47 |
30.33 |
0.86 |
| 2 |
Henderson, Brooke C (10)
|
NCGCY |
32.42 |
33.41 |
0.99 |
| 3 |
Hill, Elizabeth A (10)
|
NCGCY |
33.62 |
33.47 |
-0.15 |
| 4 |
Upton, Camryn M (9)
|
NCGCY |
32.42 |
34.19 |
1.77 |
| 5 |
Crutchfield, Lucy M (9)
|
NCGCY |
33.11 |
34.25 |
1.14 |
| 6 |
Kasa, Keira L (9)
|
NCGCY |
34.34 |
35.12 |
0.78 |
| 7 |
Todd, Lilly K (9)
|
NCGCY |
35.35 |
35.27 |
-0.08 |
| 8 |
Horan, Olivia G (10)
|
NCGCY |
|
35.63 |
- |
| 9 |
Masneri, Olivia C (10)
|
NCGCY |
38.70 |
36.54 |
-2.16 |
| 10 |
Pryor, Margot May W (10)
|
NCRACY |
|
39.14 |
- |
| 11 |
Hawkins, Charlotte J (10)
|
NCRACY |
39.39 |
40.51 |
1.12 |
| 12 |
Pearce, Keller E (9)
|
NCGCY |
43.30 |
42.85 |
-0.45 |
| 13 |
Beuhring, Anna E (9)
|
NCGCY |
40.51 |
43.45 |
2.94 |
| 14 |
Alcantara, Ximena (9)
|
NCFAST |
1:20.70 |
46.34 |
-34.36 |
| 15 |
Early, Emma E (9)
|
NCGCY |
50.63 |
46.37 |
-4.26 |
| 16 |
Gaukstern, Josephine M (10)
|
NCRACY |
48.50 |
47.04 |
-1.46 |
| 17 |
Verdin, Adilyn M (9)
|
NCRACY |
55.31 |
48.86 |
-6.45 |
| 18 |
Campbell, Avis D (10)
|
NCGCY |
58.39 |
57.16 |
-1.23 |