| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Herlocker, Patterson L (12)
|
NCRACY |
32.70 |
32.17 |
-0.53 |
| 2 |
Weaver, Emma M (12)
|
NCGCY |
39.29 |
32.94 |
-6.35 |
| 3 |
Ervin, Nadia I (12)
|
NCRACY |
33.35 |
33.47 |
0.12 |
| 4 |
Lopez, Emilia (12)
|
NCRACY |
|
33.73 |
- |
| 5 |
Beuhring, Adelaide E (11)
|
NCGCY |
34.60 |
34.10 |
-0.50 |
| 6 |
Sechriest, Emory G (11)
|
NCRACY |
34.80 |
35.02 |
0.22 |
| 7 |
Budzisch, Elise G (12)
|
NCRACY |
36.19 |
35.63 |
-0.56 |
| 8 |
Goddard, Kyla J (11)
|
NCGCY |
34.03 |
36.28 |
2.25 |
| 9 |
Dettinger, Marissa R (11)
|
NCGCY |
39.86 |
40.34 |
0.48 |
| 10 |
Friend, Katelyn M (11)
|
NCGCY |
46.02 |
42.57 |
-3.45 |
| 11 |
Riveria, Victoria S (11)
|
NCFAST |
45.45 |
43.53 |
-1.92 |