| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Gregory, Mary G (13)
|
VAWAC |
2:26.26 |
2:34.75 |
8.49 |
| 2 |
McGonigal, Maeren R (14)
|
NCGCY |
2:38.23 |
2:35.52 |
-2.71 |
| 3 |
Whitt, Jadyn B (14)
|
NCSGSA |
2:40.27 |
2:41.27 |
1.00 |
| 4 |
Cox, Delaney R (14)
|
VACGBD |
2:42.47 |
2:42.06 |
-0.41 |
| 5 |
Osborne, Hadley P (14)
|
NCGCY |
2:36.12 |
2:44.83 |
8.71 |
| 6 |
Butler, Kendall A (13)
|
VACGBD |
2:37.48 |
2:48.28 |
10.80 |
| 7 |
Miller, Scarlett I (13)
|
VAWAC |
2:40.51 |
2:49.07 |
8.56 |
| 8 |
Hanson, Wyllo C (14)
|
VACGBD |
2:53.03 |
2:50.55 |
-2.48 |
| 9 |
Hanks, Lindsay N (14)
|
VAWAC |
2:54.92 |
2:52.03 |
-2.89 |
| 10 |
Kennedy, Laura K (13)
|
NCTYDE |
2:52.34 |
2:53.42 |
1.08 |
| 11 |
Jones, Eliza A (13)
|
VACGBD |
2:59.33 |
2:56.97 |
-2.36 |
| 12 |
Wiles, Morgan E (14)
|
NCTYDE |
3:13.31 |
2:58.56 |
-14.75 |
| 12 |
Brown, Keegan H (13)
|
NCTYDE |
2:57.49 |
2:58.56 |
1.07 |
| 14 |
Teague, Helena R (14)
|
NCUN |
|
2:58.70 |
- |
| 15 |
McDonald, Anna G (13)
|
NCTYDE |
2:59.85 |
3:05.72 |
5.87 |
| 16 |
Whitworth, Margaret E (14)
|
NCTYDE |
3:15.16 |
3:08.50 |
-6.66 |
| 17 |
Yonce, Claire E (13)
|
NCSGSA |
3:19.16 |
3:08.61 |
-10.55 |
| 18 |
Moehrke, Taylor M (14)
|
NCTYDE |
2:54.61 |
3:09.07 |
14.46 |
| 19 |
Johnson, Emma-Claire F (14)
|
NCSGSA |
3:31.07 |
3:10.37 |
-20.70 |
| 20 |
Townsend, Madison S (13)
|
VAWAC |
3:07.39 |
3:11.08 |
3.69 |
| 21 |
Scott, Ashtyn H (14)
|
NCGCY |
|
3:12.12 |
- |
| 22 |
Copeland, Addison O (13)
|
NCTYDE |
3:13.82 |
3:13.26 |
-0.56 |
| 23 |
Post, Maria A (13)
|
NCTYDE |
|
3:14.15 |
- |
| 24 |
Bontrager, Allison N (13)
|
NCTYDE |
|
3:17.51 |
- |
|
Torres, Arianne C (13)
|
VAWAC |
2:51.19 |
NS |
- |
|
Williams, Jordan G (13)
|
NCGCY |
2:44.06 |
NS |
- |