| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
McDevitt, Elizabeth R (12)
|
NCYBAC |
2:25.55 |
2:25.95 |
0.40 |
| 2 |
Koh, Jenna E (12)
|
NCUN-0 |
2:27.34 |
2:29.77 |
2.43 |
| 3 |
Horn, Caitlin S (12)
|
NCEAC |
2:29.85 |
2:33.46 |
3.61 |
| 4 |
Covington, Sadie M (12)
|
NCSTAR |
2:33.95 |
2:33.93 |
-0.02 |
| 5 |
Duckworth, Heather M (12)
|
NCEAC |
2:36.26 |
2:34.58 |
-1.68 |
| 6 |
Cruz, Agnes E (11)
|
NCUN-0 |
2:23.69 |
2:34.66 |
10.97 |
| 7 |
Henderson, Abigail E (12)
|
NCSTAR |
2:40.86 |
2:41.93 |
1.07 |
| 8 |
Klein, Greta H (12)
|
NCWST |
2:50.89 |
2:44.46 |
-6.43 |
| 9 |
Haines, Madison A (12)
|
NCUN-0 |
3:12.60 |
2:47.16 |
-25.44 |
| 10 |
Scott, Lindsay A (12)
|
NCWST |
2:53.97 |
2:47.34 |
-6.63 |
| 11 |
McCurdy, Emma E (12)
|
NCTRY |
2:45.45 |
2:49.47 |
4.02 |
| 12 |
Teague, Helena R (11)
|
NCUN-0 |
|
2:51.34 |
- |
| 13 |
Manning, Skylar R (11)
|
NCSTAR |
|
2:52.47 |
- |
| 14 |
Kim, Catherine E (11)
|
NCEAC |
2:51.32 |
2:53.09 |
1.77 |
| 15 |
Walls, Kate G (11)
|
NCEAC |
|
2:55.56 |
- |
| 16 |
Roberts, Sydney E (11)
|
NCSTAR |
|
2:57.81 |
- |
| 17 |
Whitehead-Price, Lyndsey C (12)
|
NCWST |
|
3:01.71 |
- |
| 18 |
Hicks, Sarah C (12)
|
NCUN-0 |
3:16.25 |
3:03.66 |
-12.59 |
| 19 |
Perez, Olivia A (12)
|
NCSTAR |
3:41.85 |
3:05.37 |
-36.48 |
| 20 |
Stevens, Emily L (12)
|
NCSTAR |
3:05.23 |
3:12.14 |
6.91 |
| 21 |
Mathews, Laya A (11)
|
NCSTAR |
|
3:59.34 |
- |
|
Lowe, Natalie E (12)
|
NCEAC |
|
DQ |
- |