| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Muir, Vaughn K (14)
|
NCEAC |
51.62 |
49.45 |
-2.17 |
| 2 |
Mitchell, Micah J (15)
|
NCEAC |
51.32 |
50.60 |
-0.72 |
| 3 |
Abernathy, Harris S (15)
|
NCEAC |
52.88 |
51.23 |
-1.65 |
| 4 |
Sidden, Wyatt R (15)
|
NCEAC |
53.47 |
53.40 |
-0.07 |
| 5 |
Koo, Anthony J (16)
|
NCSTAR |
1:01.57 |
1:00.93 |
-0.64 |
| 6 |
Saslow, Benjamin S (11)
|
NCSFSC |
1:13.32 |
1:10.33 |
-2.99 |
| 7 |
Stevenson, Nolan A (13)
|
NCEAC |
|
1:12.04 |
- |
| 8 |
Kaper, Luke G (10)
|
NCEAC |
1:15.99 |
1:15.90 |
-0.09 |
| 9 |
Townsend, Joshua R (10)
|
NCSTAR |
1:20.78 |
1:16.14 |
-4.64 |
| 10 |
Omoruyi, Avery O (9)
|
NCEAC |
1:32.54 |
1:18.06 |
-14.48 |
| 11 |
Colon, Brayden R (10)
|
NCEAC |
1:18.16 |
1:19.85 |
1.69 |
| 12 |
Vary, Andrew M (10)
|
NCEAC |
1:34.47 |
1:31.30 |
-3.17 |
| 13 |
von der Lippe, Gabriel W (9)
|
NCSTAR |
1:39.81 |
1:33.47 |
-6.34 |
| 14 |
Eggleston, Henry R (9)
|
NCSFSC |
1:37.63 |
1:36.44 |
-1.19 |
| 15 |
Duggan, Caleb R (9)
|
NCEAC |
1:35.73 |
1:47.78 |
12.05 |
|
Otey, Hayden S (14)
|
NCEAC |
1:00.69 |
NS |
- |
|
Allison, Alexander J (12)
|
NCEAC |
1:06.35 |
NS |
- |