| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Sutherland, Ryan J (10)
|
NCYOTA |
44.11 |
44.06 |
-0.05 |
| 2 |
Jennings, Curry L (10)
|
NCYOTA |
46.34 |
45.20 |
-1.14 |
| 3 |
Hawthorne, Maxwell S (10)
|
NCWOW |
44.94 |
45.63 |
0.69 |
| 4 |
Petruzzi, Cooper J (9)
|
NCTYDE |
54.19 |
47.46 |
-6.73 |
| 5 |
Clark, Jameson W (10)
|
VASFY |
56.70 |
48.76 |
-7.94 |
| 6 |
Zeng, Colin Y (10)
|
NCYOTA |
47.38 |
48.77 |
1.39 |
| 7 |
Masneri, Ryan M (10)
|
NCGCY |
49.01 |
48.92 |
-0.09 |
| 8 |
Panol, Omar M (9)
|
NCYOTA |
52.09 |
49.79 |
-2.30 |
| 9 |
Singletary, Cohen P (10)
|
NCLTNC |
50.09 |
50.22 |
0.13 |
| 10 |
McFarland, Scott C (10)
|
NCWOW |
59.88 |
50.81 |
-9.07 |
| 11 |
Hipps, Eamon W (10)
|
NCGCY |
51.23 |
51.42 |
0.19 |
| 12 |
Weaver, Brett W (10)
|
NCSAIL |
|
52.11 |
- |
| 13 |
Mizuba, Max D (9)
|
VASFY |
52.53 |
52.95 |
0.42 |
| 14 |
Jackson, Davis T (9)
|
NCYOTA |
55.81 |
53.20 |
-2.61 |
| 15 |
Wehe, Isaiah J (9)
|
NCGCY |
53.70 |
53.87 |
0.17 |
| 16 |
Lee, Truman R (10)
|
NCSAIL |
52.99 |
53.90 |
0.91 |
| 17 |
Fullagar, Keegan H (10)
|
NCGCY |
1:05.99 |
55.84 |
-10.15 |
| 18 |
Butner, Stephen A (10)
|
NCWOW |
53.94 |
56.09 |
2.15 |
| 19 |
Pendleton, Zackary A (10)
|
NCGFSC |
56.80 |
57.78 |
0.98 |
| 20 |
Walcott, Noah S (9)
|
NCGFSC |
1:06.05 |
57.87 |
-8.18 |
| 21 |
Papaioannou, Stefanos (9)
|
NCTYDE |
1:03.72 |
58.80 |
-4.92 |
| 22 |
Sharpe, Hudson C (8)
|
NCGCY |
58.25 |
59.79 |
1.54 |
| 23 |
Moffitt, Levi R (9)
|
NCSAIL |
1:02.59 |
1:00.23 |
-2.36 |
| 24 |
Hanson, Jack C (10)
|
NCWOW |
1:00.58 |
1:00.36 |
-0.22 |
| 25 |
Gaillard, Parker J (9)
|
NCTYDE |
1:03.28 |
1:02.38 |
-0.90 |
| 26 |
Drake, Liam K (9)
|
NCRSA |
1:02.29 |
1:02.63 |
0.34 |
| 27 |
Zhang, Chuanqi (10)
|
NCYOTA |
|
1:03.24 |
- |
| 28 |
Simmons, Roman S (8)
|
NCGCY |
1:04.38 |
1:05.02 |
0.64 |
| 29 |
Meck, Wyatt D (8)
|
NCSGSA |
1:05.05 |
1:07.00 |
1.95 |
|
Murgai, Rahul A (9)
|
NCRSA |
|
DQ |
- |
|
Abebe, Yaphet B (10)
|
NCGFSC |
|
NS |
- |