| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Borden, Max W (12)
|
NCEAC |
35.52 |
35.10 |
-0.42 |
| 2 |
Williams, Henry A (12)
|
NCEAC |
34.87 |
35.62 |
0.75 |
| 3 |
Lovati, Jake L (12)
|
NCEAC |
37.53 |
36.68 |
-0.85 |
| 4 |
Hernandez-Rodriguez, Marco A (11)
|
NCEAC |
38.75 |
37.98 |
-0.77 |
| 5 |
Dickenson, Anderson D (12)
|
NCEAC |
|
38.46 |
- |
| 6 |
Hampton, Ramsey M (12)
|
NCEAC |
38.85 |
38.88 |
0.03 |
| 7 |
Urtis, Arif (12)
|
NCEAC |
40.73 |
38.98 |
-1.75 |
| 8 |
Trivette, William T (12)
|
NCNEAL |
|
41.53 |
- |
| 9 |
Hicks, Kolbe S (11)
|
NCNEAL |
44.27 |
45.97 |
1.70 |
| 10 |
Wesson, Cole T (11)
|
NCNEAL |
|
46.61 |
- |
| 11 |
Jones, Henry M (11)
|
NCNEAL |
46.85 |
46.80 |
-0.05 |
| 12 |
Hayes, Hudson R (11)
|
NCNEAL |
|
48.02 |
- |
| 13 |
Bloomfield, Asa G (11)
|
NCUN-1 |
45.74 |
48.22 |
2.48 |
| 14 |
Marahatta, Sauray (11)
|
NCPAST |
|
55.78 |
- |
| 15 |
Neal, Beckett M (11)
|
NCNEAL |
|
58.34 |
- |
|
Sutton, Nathan (11)
|
NCNEAL |
1:00.38 |
NS |
- |