| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Boyd, Sierra E (10)
|
NCTEAM |
2:29.86 |
2:26.46 |
-3.40 |
| 2 |
Winardi, Madison A (10)
|
NCMSA |
2:37.32 |
2:32.10 |
-5.22 |
| 3 |
Teague, Avalon G (10)
|
NCSTAR |
2:34.53 |
2:35.80 |
1.27 |
| 4 |
McCormick, Gillian R (10)
|
NCSMAC |
2:43.91 |
2:41.45 |
-2.46 |
| 5 |
Kong, Kaia A (9)
|
NCMSA |
2:45.52 |
2:41.95 |
-3.57 |
| 6 |
Spencer, Addison M (10)
|
NCHPSC |
2:57.91 |
2:43.65 |
-14.26 |
| 7 |
Picard, Avery E (10)
|
NCTEAM |
2:48.41 |
2:45.75 |
-2.66 |
| 8 |
Carpenter, Ellory K (10)
|
NCMSA |
2:53.24 |
2:48.77 |
-4.47 |
| 9 |
Starkey, Rachel E (9)
|
NCEAC |
2:58.67 |
2:52.75 |
-5.92 |
| 10 |
Martin, Avery G (10)
|
NCMSA |
2:54.03 |
2:53.32 |
-0.71 |
| 11 |
Stevens, Katherine S (9)
|
NCSTAR |
3:01.47 |
2:55.84 |
-5.63 |
| 12 |
Giersch, Tessa E (10)
|
NCMSA |
2:56.66 |
2:57.53 |
0.87 |
| 13 |
Singleton, Julianna M (9)
|
NCSTAR |
3:08.16 |
3:01.84 |
-6.32 |
| 14 |
Singh, Anjali S (10)
|
NCTEAM |
|
3:04.28 |
- |
| 15 |
Shepherd, Sarah C (10)
|
NCASTF |
|
3:07.51 |
- |
|
Lothakoun, Rachel L (10)
|
NCSAIL |
2:55.71 |
NS |
- |
|
Kim, Elizabeth M (10)
|
NCEAC |
2:57.55 |
NS |
- |