| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Claborn, Alyssa A (10)
|
NCLOY |
33.55 |
33.89 |
0.34 |
| 2 |
Betancourt, Amelia H (10)
|
NCLOY |
37.04 |
34.95 |
-2.09 |
| 3 |
Bellissimo, Sophia G (10)
|
NCLOY |
36.28 |
35.52 |
-0.76 |
| 4 |
McDonough, Addyson R (10)
|
NCLOY |
40.83 |
43.61 |
2.78 |
| 5 |
Filiault, Julia K (10)
|
NCLOY |
45.39 |
45.41 |
0.02 |
| 6 |
Wiechers, Abigail F (10)
|
NCLOY |
|
46.71 |
- |
| 7 |
Lawrence, Amelia S (10)
|
NCWA |
46.66 |
47.48 |
0.82 |
| 8 |
Carpenter, Addison L (10)
|
NCLOY |
|
49.05 |
- |
| 9 |
Allen, Grace M (10)
|
NCLOY |
|
50.43 |
- |
| 10 |
Hoots, Olivia L (10)
|
NCGCY |
|
57.16 |
- |
| 11 |
Dingman, Kasey S (9)
|
NCGCY |
|
58.63 |
- |
| 12 |
Grubb, Emma M (9)
|
NCGCY |
1:17.08 |
1:01.48 |
-15.60 |
| 13 |
McGuire, Molly S (10)
|
NCLOY |
|
1:04.25 |
- |
|
Waters, Londyn S (9)
|
NCGCY |
1:05.88 |
DQ |
- |
|
Misener, Leah J (10)
|
NCLOY |
36.00 |
NS |
- |