| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Najera, Aileen F (8)
|
NCHPSC |
35.47 |
33.87 |
-1.60 |
| 2 |
Ramseur, Riley G (7)
|
NCSFSC |
40.99 |
37.65 |
-3.34 |
| 3 |
Norris, Madison R (8)
|
NCTEAM |
41.62 |
40.13 |
-1.49 |
| 4 |
Gardner, Gabrielle N (7)
|
NCHPSC |
46.37 |
42.34 |
-4.03 |
| 5 |
Najera, Lindsay L (6)
|
NCHPSC |
43.80 |
42.90 |
-0.90 |
| 6 |
LaRue, Bailey A (8)
|
NCHPSC |
37.99 |
43.59 |
5.60 |
| 7 |
Luedke, Tatum M (7)
|
NCTEAM |
44.11 |
44.29 |
0.18 |
| 8 |
Maley, Samantha C (7)
|
NCSFSC |
54.24 |
56.95 |
2.71 |
| 9 |
Reynolds, Lily C (7)
|
NCSFSC |
1:01.79 |
1:01.17 |
-0.62 |
| 10 |
Smith, Emma R (7)
|
NCSFSC |
1:03.58 |
1:04.56 |
0.98 |
| 11 |
Maley, Sara C (6)
|
NCSFSC |
1:13.20 |
1:12.93 |
-0.27 |
|
Creel, Kinsley E (8)
|
NCSSS |
|
NS |
- |
|
Krumroy, Ainsley E (8)
|
NCSFSC |
59.51 |
NS |
- |
|
Saunders, Grayson-Ann P (8)
|
NCSFSC |
|
NS |
- |