| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Smith, Lauron K (9)
|
NCSTAR |
22.06 |
19.26 |
-2.80 |
| 2 |
Stevens, Katherine S (8)
|
NCSTAR |
19.75 |
20.13 |
0.38 |
| 3 |
Perez-Romero, Yatzil E (10)
|
NCSTAR |
|
22.18 |
- |
| 4 |
Willis, Mary E (7)
|
NCSFSC |
25.99 |
22.32 |
-3.67 |
| 5 |
Smith, McKenzie G (9)
|
NCSTAR |
24.71 |
22.46 |
-2.25 |
| 6 |
Sakalouskaya, Valerie G (8)
|
NCSTAR |
|
23.11 |
- |
| 7 |
Conklin, Highland S (8)
|
NCSFSC |
24.78 |
23.92 |
-0.86 |
| 8 |
Scott, Isabella B (8)
|
NCYBAC |
|
24.39 |
- |
| 9 |
McDonald, Mallory M (7)
|
NCSFSC |
30.33 |
24.58 |
-5.75 |
| 10 |
Wieting, Reagan J (9)
|
NCSTAR |
29.75 |
25.27 |
-4.48 |
| 11 |
Coyne, Morgan I (8)
|
NCSTAR |
26.23 |
25.32 |
-0.91 |
| 12 |
Jorgenson, Emily A (6)
|
NCSFSC |
|
25.44 |
- |
| 13 |
Morgan, Eve K (8)
|
NCSFSC |
28.64 |
25.71 |
-2.93 |
| 14 |
Fulton, Charley G (9)
|
NCSFSC |
31.93 |
26.06 |
-5.87 |
| 15 |
Matthews, Ashton A (7)
|
NCSFSC |
28.78 |
26.13 |
-2.65 |
| 16 |
Cobb, Chloe A (8)
|
NCSFSC |
|
26.23 |
- |
| 17 |
Teague, Rowan C (6)
|
NCSTAR |
28.21 |
26.47 |
-1.74 |
| 18 |
Richardson, Sabina R (10)
|
NCSFSC |
|
26.59 |
- |
| 19 |
Maley, Sara C (8)
|
NCSFSC |
31.17 |
30.95 |
-0.22 |
| 20 |
Kunz, Annabelle L (4)
|
NCSFSC |
|
34.01 |
- |
| 21 |
Jorgenson, Ella A (5)
|
NCSFSC |
|
35.38 |
- |
| 22 |
Weber, Ryley M (6)
|
NCYBAC |
|
37.28 |
- |
| 23 |
Gilbert, Madelyn R (6)
|
NCSFSC |
|
38.74 |
- |
| 24 |
Ward, Maddison L (6)
|
NCYBAC |
|
39.51 |
- |