| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Hering, Tanner A (18)
|
NCSGSA |
51.29 |
53.45 |
2.16 |
| 2 |
Keener, Noah K (16)
|
NCEAC |
54.30 |
54.89 |
0.59 |
| 3 |
Cantrell, Eric J (17)
|
NCSGSA |
52.63 |
55.45 |
2.82 |
| 4 |
Watson, William C (17)
|
NCSTAR |
55.21 |
56.60 |
1.39 |
| 5 |
Keaney, Reilly E (16)
|
NCSGSA |
54.64 |
56.87 |
2.23 |
| 6 |
Mikuta, Christopher R (18)
|
NCSGSA |
53.85 |
58.02 |
4.17 |
| 7 |
Maycock, Julian L (16)
|
NCSGSA |
58.70 |
59.07 |
0.37 |
| 8 |
Walls, Harrison R (16)
|
NCEAC |
57.51 |
59.50 |
1.99 |
| 9 |
Holian, Tanner J (14)
|
NCSGSA |
58.16 |
59.58 |
1.42 |
| 9 |
Brown, Logan R (16)
|
NCEAC |
1:00.83 |
59.58 |
-1.25 |
| 11 |
Hering, Flinn M (16)
|
NCSGSA |
56.86 |
1:00.08 |
3.22 |
| 12 |
Smelzer, Albert M (12)
|
NCSGSA |
1:01.98 |
1:00.92 |
-1.06 |
| 13 |
Cartagena, Aidan C (13)
|
NCEAC |
1:01.97 |
1:01.54 |
-0.43 |
| 14 |
Schilling, Christopher A (13)
|
NCSGSA |
1:02.47 |
1:02.18 |
-0.29 |
| 15 |
Austin, Thaddeus E (12)
|
NCEAC |
1:12.28 |
1:02.24 |
-10.04 |
| 16 |
Glebus, Christopher P (15)
|
NCSGSA |
1:02.64 |
1:03.87 |
1.23 |
| 17 |
Brooks, Nicholas R (11)
|
NCEAC |
1:07.79 |
1:07.98 |
0.19 |
| 18 |
Guthrie, Reade A (10)
|
NCSGSA |
1:09.17 |
1:08.88 |
-0.29 |
| 19 |
Mann, Spencer J (13)
|
NCEAC |
1:07.96 |
1:09.29 |
1.33 |
| 20 |
Teoh, Reid P (14)
|
NCSTAR |
1:11.34 |
1:12.02 |
0.68 |
| 21 |
Hudson, Puckett W (10)
|
NCXA |
|
1:17.29 |
- |
| 22 |
Mitchell, Micah J (11)
|
NCEAC |
1:16.56 |
1:19.98 |
3.42 |
| 23 |
Nunn, Bo B (9)
|
NCEAC |
1:25.98 |
1:22.99 |
-2.99 |
| 24 |
Clark, Max H (10)
|
NCXA |
|
1:23.02 |
- |
| 25 |
Pfeifer, Evan H (12)
|
NCSTAR |
1:39.09 |
1:24.91 |
-14.18 |
| 26 |
Powell, David A (12)
|
NCEAC |
1:27.32 |
1:25.33 |
-1.99 |
| 27 |
Teoh, Wesley P (11)
|
NCSTAR |
2:03.80 |
1:27.07 |
-36.73 |
| 28 |
Willis, Berkley S (10)
|
NCEAC |
1:28.27 |
1:35.04 |
6.77 |
|
Leone, Peter N (10)
|
NCEAC |
1:54.14 |
DQ |
- |
|
Schall, James M (12)
|
NCUN-0 |
1:27.85 |
DQ |
- |
|
Smelzer, Peter B (16)
|
NCSGSA |
55.81 |
NS |
- |