| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Michalowicz, Nathan C (12)
|
NCECA |
25.09 |
24.87 |
-0.22 |
| 2 |
Zapler, Ezra N (11)
|
NCECA |
25.49 |
25.31 |
-0.18 |
| 3 |
Hughes, Jackson C (12)
|
NCWOW |
26.53 |
26.22 |
-0.31 |
| 4 |
Arredondo, Nathan B (12)
|
NCWOW |
28.94 |
26.40 |
-2.54 |
| 5 |
Camacho, Clement D (11)
|
NCWOW |
26.82 |
26.56 |
-0.26 |
| 6 |
Hawthorne, Maxwell S (11)
|
NCWOW |
28.71 |
26.70 |
-2.01 |
| 7 |
Reiniche, Ryan J (11)
|
NCWOW |
29.49 |
28.90 |
-0.59 |
| 8 |
Andrews, Brycen M (11)
|
NCECA |
30.16 |
29.63 |
-0.53 |
| 9 |
Mejouev, Maxim (12)
|
NCWOW |
31.35 |
30.30 |
-1.05 |
| 10 |
Butner, Stephen A (11)
|
NCWOW |
31.72 |
31.17 |
-0.55 |
| 11 |
Burckhardt, Ayden C (12)
|
NCHCAC |
31.68 |
31.34 |
-0.34 |
| 12 |
Grimshaw, Alexander L (12)
|
NCSSS |
31.58 |
31.35 |
-0.23 |
| 13 |
Bollenbacher, Stephen W (12)
|
NCWOW |
32.81 |
31.69 |
-1.12 |
| 14 |
Bullard, Cameron E (11)
|
NCECA |
31.47 |
31.75 |
0.28 |
| 15 |
Johnson, Samuel T (11)
|
NCECA |
32.52 |
32.19 |
-0.33 |
| 16 |
Bullard, Andrew W (11)
|
NCECA |
33.24 |
32.27 |
-0.97 |
| 17 |
Lowry, Noah R (11)
|
NCHCAC |
45.00 |
33.09 |
-11.91 |
| 18 |
Jenkins, Owen C (11)
|
NCHCAC |
33.43 |
34.03 |
0.60 |
| 19 |
Horsman, Cody R (11)
|
NCHCAC |
36.37 |
35.06 |
-1.31 |
| 20 |
Gedman, Isaac N (11)
|
NCBAC |
41.60 |
40.67 |
-0.93 |
| 21 |
Blische, Colby G (11)
|
NCHCAC |
55.00 |
50.21 |
-4.79 |
|
Anderson, Cole B (11)
|
NCECA |
36.50 |
DQ |
- |
|
Chen, Jack (12)
|
NCECA |
|
NS |
- |
|
Roth, Matej E (12)
|
NCECA |
27.02 |
NS |
- |