| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Lee, Gabriella S (8)
|
NCSSS |
24.38 |
25.16 |
0.78 |
| 2 |
Stegman, Peyton N (8)
|
NCBAC |
30.43 |
25.47 |
-4.96 |
| 3 |
Nicholson, Madelyn G (8)
|
NCWOW |
25.16 |
25.55 |
0.39 |
| 4 |
Milkowich, Alayna G (8)
|
NCSSS |
27.17 |
26.17 |
-1.00 |
| 5 |
Brewer, Reagan N (7)
|
NCRMY |
29.28 |
26.68 |
-2.60 |
| 6 |
Senter, Ainsley J (8)
|
NCCFAC |
27.10 |
26.71 |
-0.39 |
| 7 |
Kibbe, Elizabeth E (8)
|
NCRMY |
27.62 |
26.99 |
-0.63 |
| 8 |
Savage, Margaret C (7)
|
NCWOW |
31.18 |
30.88 |
-0.30 |
| 9 |
Lee, Delilah K (6)
|
NCSSS |
35.56 |
34.46 |
-1.10 |
| 10 |
Kelley, Cecelia F (8)
|
NCECA |
36.14 |
35.06 |
-1.08 |
| 11 |
Garver, Meghan E (8)
|
NCBAC |
|
40.17 |
- |
| 12 |
Gillikin, Millicent E (7)
|
NCECA |
|
44.92 |
- |
| 13 |
Kibbe, Rebekah R (7)
|
NCRMY |
32.39 |
45.05 |
12.66 |
| 14 |
McMullan, Annabelle B (4)
|
NCBAC |
|
57.66 |
- |
|
Burckhardt, Adyson C (8)
|
NCHCAC |
36.17 |
DQ |
- |
|
Raju, Irah N (7)
|
NCECA |
|
DQ |
- |
|
Pake, Anna B (8)
|
NCECA |
28.99 |
DQ |
- |
|
Leggett, Katy B (7)
|
NCECA |
|
DQ |
- |
|
Lee, Eliza S (7)
|
NCECA |
|
DQ |
- |
|
Richardson, Mattie S (6)
|
NCECA |
|
NS |
- |
|
Paylor, Kaleigh E (6)
|
NCECA |
|
NS |
- |
|
Carroll, Charlotte A (8)
|
NCWOW |
29.28 |
NS |
- |