| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Stuck, Kelsey M (12)
|
NCWOW |
29.80 |
28.68 |
-1.12 |
| 2 |
Crane, Taylor M (11)
|
NCWOW |
30.55 |
29.88 |
-0.67 |
| 3 |
Rountree, Finley S (12)
|
NCWOW |
31.29 |
31.96 |
0.67 |
| 4 |
Gaskill, Meredith R (12)
|
NCECA |
35.16 |
32.39 |
-2.77 |
| 5 |
Reed, Elizabeth R (12)
|
NCECA |
32.61 |
32.52 |
-0.09 |
| 6 |
Williams, Lexi M (12)
|
NCWOW |
31.62 |
32.76 |
1.14 |
| 7 |
Weigand, Kelsey C (12)
|
NCWOW |
33.49 |
33.28 |
-0.21 |
| 8 |
Seay, Caroline H (12)
|
NCWOW |
34.96 |
33.41 |
-1.55 |
| 9 |
Hughes, Chloe A (11)
|
NCWOW |
34.74 |
33.60 |
-1.14 |
| 10 |
Bollenbacher, Heidi F (11)
|
NCWOW |
34.58 |
34.13 |
-0.45 |
| 11 |
Santucci, Skylar (12)
|
NCWOW |
35.12 |
36.08 |
0.96 |
| 12 |
Wert, Sophia G (11)
|
NCWOW |
38.19 |
37.23 |
-0.96 |
| 13 |
Carroll, Mackenzie M (11)
|
NCWOW |
37.67 |
38.69 |
1.02 |
| 14 |
Hoey, Parker J (11)
|
NCWOW |
48.73 |
39.97 |
-8.76 |
| 15 |
Moore, Gracie C (11)
|
NCECA |
41.14 |
40.05 |
-1.09 |
| 16 |
Sterner, Adalyn G (12)
|
NCWOW |
40.23 |
43.65 |
3.42 |
| 17 |
Laird, Helena E (11)
|
NCECA |
38.61 |
44.56 |
5.95 |
| 18 |
Leggett, Ava V (11)
|
NCECA |
45.25 |
44.72 |
-0.53 |
| 19 |
Chen, Lina (12)
|
NCECA |
|
48.79 |
- |
|
Smith, Clara P (11)
|
NCECA |
34.89 |
NS |
- |
|
Mitchell, Caroline G (11)
|
NCECA |
|
NS |
- |
|
Reaves, Camren G (12)
|
NCWOW |
33.70 |
NS |
- |