| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Lee, Gabriella S (8)
|
NCSSS |
56.03 |
50.98 |
-5.05 |
| 2 |
Nicholson, Madelyn G (8)
|
NCWOW |
55.94 |
54.49 |
-1.45 |
| 3 |
Senter, Ainsley J (8)
|
NCCFAC |
1:07.33 |
56.17 |
-11.16 |
| 4 |
Brewer, Reagan N (7)
|
NCRMY |
1:00.89 |
59.41 |
-1.48 |
| 5 |
Pake, Anna B (8)
|
NCECA |
1:06.10 |
1:01.48 |
-4.62 |
| 6 |
Savage, Margaret C (7)
|
NCWOW |
1:01.22 |
1:05.48 |
4.26 |
| 7 |
McLean, Claire R (8)
|
NCWOW |
1:06.70 |
1:05.61 |
-1.09 |
| 8 |
Lee, Delilah K (6)
|
NCSSS |
1:13.05 |
1:07.46 |
-5.59 |
| 9 |
Boyce, Audri P (8)
|
NCECA |
1:05.37 |
1:08.88 |
3.51 |
| 10 |
Hill, Reese H (8)
|
NCECA |
1:15.31 |
1:11.89 |
-3.42 |
| 11 |
Burckhardt, Adyson C (8)
|
NCHCAC |
1:05.00 |
1:18.98 |
13.98 |
|
Garver, Meghan E (8)
|
NCBAC |
1:16.31 |
DQ |
- |
|
Gillikin, Millicent E (7)
|
NCECA |
|
DQ |
- |
|
Spannhake, Lexie J (7)
|
NCHCAC |
1:15.00 |
DQ |
- |
|
Kibbe, Rebekah R (7)
|
NCRMY |
1:15.65 |
DQ |
- |
|
Cumbee, Phoebe A (8)
|
NCBAC |
1:15.12 |
NS |
- |