| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Mattle, Anthony D (20)
|
SNDART |
25.02 |
24.87 |
-0.15 |
| 2 |
Musabekov, Daniiar D (17)
|
CACCAT |
25.50 |
25.06 |
-0.44 |
| 3 |
Holton, Stuart M (17)
|
CANOVA |
25.22 |
25.13 |
-0.09 |
| 4 |
Ballard, Jacob A (16)
|
IESWAC |
25.04 |
25.32 |
0.28 |
| 5 |
Sevidal, Ryan K (16)
|
CAPATH |
25.14 |
25.70 |
0.56 |
| 6 |
Sorenson, Matthew R (18)
|
CASET |
25.49 |
25.73 |
0.24 |
| 7 |
Lipton, Ian P (15)
|
PNBISC |
26.50 |
26.02 |
-0.48 |
| 8 |
Rowen, Luke M (19)
|
CALASC |
25.76 |
26.19 |
0.43 |
| 9 |
Hickey, Molly E (17)
|
PNUPAC |
27.23 |
27.49 |
0.26 |
| 10 |
Ballenger, Marie A (32)
|
PCUN-0 |
27.00 |
27.60 |
0.60 |
| 11 |
Weiss, Jackson L (14)
|
CAALPH |
27.78 |
27.75 |
-0.03 |
| 12 |
Barner, Nicholas L (13)
|
CAALPH |
27.00 |
27.80 |
0.80 |
| 13 |
Cho, Allison Y (17)
|
CACANY |
27.70 |
27.93 |
0.23 |
| 14 |
Rodriguez, Xaveria M (17)
|
PNBISC |
27.69 |
28.09 |
0.40 |
| 15 |
Albertson, Kai R (14)
|
CAALPH |
27.52 |
28.11 |
0.59 |
| 16 |
Catig, Maxine Fidelis C (17)
|
CACANY |
28.02 |
28.18 |
0.16 |
| 17 |
Balbuena, Luna (14)
|
PCPEAK |
28.50 |
28.22 |
-0.28 |
| 18 |
Sapico, Simone A (15)
|
SNCCA |
29.47 |
28.46 |
-1.01 |
| 19 |
Holden, Audrey E (14)
|
PCPASA |
28.33 |
28.85 |
0.52 |
| 20 |
Feng, Elaine Y (16)
|
PCPEAK |
29.10 |
29.15 |
0.05 |
| 21 |
Crother-Collado, Gabriela A (15)
|
CAALPH |
29.73 |
29.55 |
-0.18 |
| 22 |
Dykstra, Lily K (15)
|
CAALPH |
29.59 |
29.69 |
0.10 |