| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Cao, Cecilia Y (12)
|
ADDELM |
41.49 |
35.16 |
-6.33 |
| 2 |
Plante, Megan G (12)
|
ADUN |
|
36.05 |
- |
| 3 |
Amedio, Mea K (11)
|
ADSCHE |
35.30 |
36.64 |
1.34 |
| 4 |
Lufkin, Sina C (12)
|
ADNTS |
40.74 |
39.55 |
-1.19 |
| 5 |
Jerabek, Ulka G (12)
|
ADDELM |
|
40.69 |
- |
| 6 |
Swan-Scott, Rowan M (11)
|
ADGLFY |
49.99 |
41.52 |
-8.47 |
| 7 |
Sellnow, Abigail V (11)
|
ADEELS |
45.58 |
41.94 |
-3.64 |
| 8 |
Barkley, Katherine E (10)
|
ADNTS |
46.46 |
43.51 |
-2.95 |
| 9 |
Myers, Myah K (12)
|
ADNTS |
45.42 |
43.54 |
-1.88 |
| 10 |
Fitzgerald, Catherine G (11)
|
ADDELM |
50.48 |
47.32 |
-3.16 |
| 11 |
Armstrong, Chloe V (11)
|
ADEELS |
55.57 |
49.52 |
-6.05 |
| 12 |
Gossett, Savannah R (12)
|
ADGLFY |
46.83 |
49.86 |
3.03 |
| 13 |
Ottoson, Midian R (12)
|
ADSIDN |
|
53.17 |
- |
| 14 |
Sellnow, Emma M (9)
|
ADEELS |
57.35 |
53.47 |
-3.88 |
| 15 |
Gossett, Willow V (10)
|
ADGLFY |
54.26 |
1:01.83 |
7.57 |
| 16 |
Carter, Gwen S (9)
|
ADEELS |
|
1:05.14 |
- |
|
Hopper, Olivia K (11)
|
ADDELM |
|
DQ |
- |