| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Shao, Emma X (8)
|
ADGUY |
53.16 |
50.49 |
-2.67 |
| 2 |
Rogowski, Isabella S (8)
|
ADSCHE |
53.26 |
52.15 |
-1.11 |
| 3 |
Braun, Julia L (8)
|
ADNTS |
1:00.98 |
57.53 |
-3.45 |
| 4 |
Allinson, Mae L (8)
|
ADDELM |
1:00.08 |
59.88 |
-0.20 |
| 5 |
Zen, Dominique A (8)
|
ADDELM |
1:01.39 |
1:00.14 |
-1.25 |
| 6 |
Zhang, Annabelle Y (8)
|
ADCPP |
1:02.18 |
1:02.19 |
0.01 |
| 7 |
Relyea, Cadence G (7)
|
ADDELM |
1:08.61 |
1:02.40 |
-6.21 |
| 8 |
Walter, Emma L (8)
|
ADCPP |
1:14.56 |
1:03.13 |
-11.43 |
| 9 |
Stoup, Madelyn L (8)
|
ADCPP |
1:07.87 |
1:03.91 |
-3.96 |
| 10 |
Stewart Fraser, Thornton R (7)
|
ADALBS |
1:03.34 |
1:05.36 |
2.02 |
|
Adams, Michelle E (7)
|
ADCPP |
1:08.78 |
DQ |
- |
|
O'Donnell, Bridgett M (8)
|
ADEELS |
1:30.34 |
NS |
- |