| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Durr, Tucker C (10)
|
ADSCHE |
49.30 |
48.02 |
-1.28 |
| 2 |
Brkanovic, Isak A (10)
|
ADDELM |
50.81 |
48.16 |
-2.65 |
| 3 |
O'Leary, Anthony U (9)
|
ADSCHE |
51.09 |
49.32 |
-1.77 |
| 4 |
Hanna, Nathan C (10)
|
ADCLIN |
56.26 |
51.85 |
-4.41 |
| 5 |
Tieckelmann, Connor R (10)
|
ADSCHE |
58.43 |
52.20 |
-6.23 |
| 6 |
VanAlst, Amon J (10)
|
ADUN |
56.52 |
53.12 |
-3.40 |
| 7 |
McNair, John P (9)
|
ADCLIN |
56.23 |
54.34 |
-1.89 |
| 8 |
Scerbo, Caleb F (10)
|
ADCLIN |
50.18 |
55.19 |
5.01 |
| 9 |
Killian, Daniel S (10)
|
ADCPP |
55.13 |
55.26 |
0.13 |
| 10 |
Rudd, Conner D (10)
|
ADCPP |
58.18 |
55.85 |
-2.33 |
| 11 |
Simmons, Cooper Z (10)
|
ADCPP |
57.92 |
57.73 |
-0.19 |
| 12 |
Wilkosz, Matthew M (10)
|
ADCLIN |
1:03.90 |
58.04 |
-5.86 |
| 13 |
Zeliph, Thomas J (9)
|
ADALBS |
1:04.60 |
59.51 |
-5.09 |
| 14 |
Staudinger, Samuel P (9)
|
ADALBS |
58.74 |
59.80 |
1.06 |
| 15 |
Wang, Jake H (10)
|
ADSCHE |
1:03.30 |
1:00.65 |
-2.65 |
| 16 |
Hadzimujic, Edin (9)
|
ADNHA |
1:06.52 |
1:05.59 |
-0.93 |
|
Cao, James Y (9)
|
ADDELM |
1:01.24 |
DQ |
- |
|
Lischinsky, Stephen J (10)
|
ADHAWK |
50.09 |
NS |
- |