| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Brkanovic, Isak A (11)
|
ADDELM |
31.08 |
36.18 |
5.10 |
| 2 |
Rudd, Conner D (12)
|
ADCPP |
41.85 |
37.38 |
-4.47 |
| 3 |
Keller, Brady C (12)
|
ADCPP |
41.24 |
37.44 |
-3.80 |
| 4 |
Hershberg, Jacob C (11)
|
ADDELM |
32.72 |
38.02 |
5.30 |
| 5 |
Zeliph, Thomas J (11)
|
ADALBS |
41.82 |
39.41 |
-2.41 |
| 6 |
VanAlst, Amon J (12)
|
ADUN |
42.86 |
41.86 |
-1.00 |
| 7 |
Gilbert, Spencer X (11)
|
ADGLEN |
44.18 |
42.64 |
-1.54 |
| 8 |
Paradis, Degaregen D (11)
|
ADCLIN |
|
43.46 |
- |
| 9 |
Staudinger, Samuel P (11)
|
ADALBS |
46.98 |
43.61 |
-3.37 |
| 10 |
Tieckelmann, Connor R (12)
|
ADUN |
45.07 |
44.11 |
-0.96 |
| 11 |
Killian, Daniel S (12)
|
ADCPP |
43.78 |
44.61 |
0.83 |
| 12 |
Hook, Gavin M (12)
|
ADCPP |
48.60 |
45.85 |
-2.75 |
| 13 |
Upton, Matthew T (12)
|
ADCPP |
47.14 |
46.16 |
-0.98 |
| 14 |
Simmons, Cooper Z (12)
|
ADCPP |
48.27 |
47.21 |
-1.06 |
| 15 |
Putnam, Parker M (11)
|
ADCLIN |
|
47.65 |
- |
| 16 |
Guzewich, Timothy E (11)
|
ADGCAT |
41.00 |
49.33 |
8.33 |
| 17 |
Dischiavo, Luke D (11)
|
ADNHA |
52.53 |
49.87 |
-2.66 |
| 18 |
Armendariz-Huang, Daniel P (12)
|
ADNTS |
50.13 |
53.00 |
2.87 |
| 19 |
Sheaffer, Roman K (11)
|
ADCLIN |
|
53.06 |
- |
| 20 |
Ostovar, Kasper C (11)
|
ADCLRK |
48.14 |
53.12 |
4.98 |
| 21 |
Clive, Eoin T (11)
|
ADNHA |
51.48 |
53.89 |
2.41 |
| 22 |
Kinley, London D (11)
|
ADCLRK |
45.82 |
55.17 |
9.35 |
| 23 |
Deuel, Kristopher M (11)
|
ADGCAT |
41.32 |
58.48 |
17.16 |
| 24 |
Hughes Robillard, Eric M (11)
|
ADCPP |
1:07.82 |
1:16.35 |
8.53 |
| 25 |
St. Thomas, Logan M (11)
|
ADCLIN |
1:12.43 |
1:20.65 |
8.22 |
|
Wilkosz, Matthew M (12)
|
ADCLIN |
45.84 |
DQ |
- |
|
Lee, Eun Chan (11)
|
ADCPP |
41.71 |
DQ |
- |
|
Paradis, Cohen A (12)
|
ADCLIN |
|
DQ |
- |