| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Zegowitz, Mabel J (15)
|
MDBAY |
26.28 |
26.09 |
-0.19 |
| 2 |
Wooten, Hannah P (15)
|
NCRSA |
26.50 |
26.55 |
0.05 |
| 3 |
Lu, Angelina Z (15)
|
NCRSA |
26.84 |
26.78 |
-0.06 |
| 4 |
Halsey, Joslin J (16)
|
NEBGSC |
27.34 |
27.11 |
-0.23 |
| 5 |
Clattenburg, Hannah C (15)
|
PVTOLL |
27.87 |
27.15 |
-0.72 |
| 6 |
Schaaf, Alexis V (15)
|
VAGATR |
30.05 |
27.99 |
-2.06 |
| 7 |
Patel, Lily H (16)
|
PVDRAG |
29.32 |
29.33 |
0.01 |
| 8 |
Kanak, Elizabeth A (17)
|
VAHVAC |
|
29.35 |
- |
| 9 |
Merideth, Isabel J (15)
|
PVTOLL |
|
29.38 |
- |
| 10 |
Byrnes, Jia L (16)
|
VAGATR |
33.32 |
29.83 |
-3.49 |
| 11 |
Brown, Isabel D (15)
|
PVTOLL |
30.14 |
30.20 |
0.06 |
| 12 |
Musso, Morgan E (16)
|
COPCST |
29.84 |
30.65 |
0.81 |
| 13 |
Down, Isabella C (15)
|
PVTOLL |
|
30.86 |
- |
| 14 |
White, Taylor B (16)
|
PVTOLL |
30.48 |
31.80 |
1.32 |
| 15 |
Ward, Lily E (15)
|
VAGATR |
34.86 |
32.22 |
-2.64 |
| 16 |
Surendorj, Nomunkhatan (15)
|
PVTOLL |
|
32.58 |
- |
| 17 |
Gooding, Elaina (17)
|
VAHVAC |
|
34.29 |
- |
| 18 |
Banks, Hannah (15)
|
VAHVAC |
|
38.03 |
- |
|
Patterson, Brooke E (15)
|
PVTOLL |
|
DQ |
- |
|
O'Malley, Ella M (9)
|
VAGATR |
|
DQ |
- |
|
Loftus, Grace K (10)
|
PVASTS |
38.70 |
NS |
- |
|
Alvarado, Anahi (16)
|
VAWST |
|
NS |
- |