| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Spurlock, Avery M (10)
|
VACCA |
30.17 |
30.38 |
0.21 |
| 2 |
Gorby, Lila (10)
|
VAGATR |
31.06 |
31.87 |
0.81 |
| 3 |
Brushwood, Madison P (10)
|
VABASS |
34.84 |
35.97 |
1.13 |
| 3 |
Riggleman, Shelby R (9)
|
VAGATR |
36.20 |
35.97 |
-0.23 |
| 5 |
Deeble, Sadie C (9)
|
VAGATR |
37.20 |
37.00 |
-0.20 |
| 6 |
McConnell, Ansley G (9)
|
VAGATR |
40.87 |
37.70 |
-3.17 |
| 7 |
Dunham, Lila A (10)
|
VAGATR |
36.79 |
37.72 |
0.93 |
| 8 |
Smallwood, Marissa R (10)
|
VAGATR |
46.32 |
39.45 |
-6.87 |
| 9 |
Mancini, Hayley A (9)
|
VAGATR |
39.00 |
39.54 |
0.54 |
| 10 |
Shafer, Naomi R (9)
|
VAGATR |
37.54 |
40.02 |
2.48 |
| 11 |
Fuentes, Camille M (9)
|
VASTRM |
39.41 |
40.50 |
1.09 |
| 12 |
Clark, Madison T (9)
|
VABASS |
|
42.95 |
- |
| 13 |
Kann, Jozelyn F (10)
|
VAGATR |
44.00 |
43.04 |
-0.96 |
| 14 |
Kullander, Jocelyn E (9)
|
VAGATR |
48.73 |
47.72 |
-1.01 |