| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Henderson, Kaden T (10)
|
AMBRY |
35.08 |
35.38 |
0.30 |
| 2 |
Zhao, Caden W (10)
|
AMPEAQ |
37.84 |
35.42 |
-2.42 |
| 3 |
Howard, Thomas A (10)
|
AMMLAC |
37.32 |
36.08 |
-1.24 |
| 4 |
O'Keefe, Logan E (9)
|
AMANSC |
38.53 |
36.11 |
-2.42 |
| 5 |
Pickett, Noah M (10)
|
AMMAC |
40.12 |
36.82 |
-3.30 |
| 6 |
Turner, Joshua O (10)
|
AMRACE |
38.29 |
37.22 |
-1.07 |
| 7 |
Punturiero, Salvatore A (10)
|
AMPEAQ |
37.87 |
37.29 |
-0.58 |
| 8 |
Morgan, Tabari A (10)
|
AMRESY |
38.70 |
38.84 |
0.14 |
| 9 |
Hankinson, Ethan J (10)
|
AMMOON |
39.14 |
38.87 |
-0.27 |
| 10 |
Morrell, Andrew M (10)
|
AMANSC |
39.40 |
39.71 |
0.31 |
| 11 |
Pickett, Christopher B (8)
|
AMMAC |
40.40 |
55.78 |
15.38 |