| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Singh, Sahil R (10)
|
AMSFAC |
33.45 |
30.83 |
-2.62 |
| 2 |
Howard, Thomas A (10)
|
AMMLAC |
33.37 |
31.11 |
-2.26 |
| 3 |
Chen, Alexander B (10)
|
AMJCCS |
31.29 |
31.17 |
-0.12 |
| 4 |
Marcincavage, Roddy G (10)
|
AMNAC |
31.53 |
31.22 |
-0.31 |
| 5 |
Powell, Caden R (10)
|
AMBPR |
32.76 |
31.77 |
-0.99 |
| 6 |
Zhao, Caden W (10)
|
AMPEAQ |
32.56 |
31.87 |
-0.69 |
| 7 |
Semenov, Ethan B (10)
|
AMJCCS |
32.47 |
32.15 |
-0.32 |
| 8 |
Morrell, Andrew M (10)
|
AMANSC |
32.59 |
32.87 |
0.28 |
| 9 |
O'Keefe, Logan E (9)
|
AMANSC |
35.72 |
33.24 |
-2.48 |
| 10 |
Pickett, Noah M (10)
|
AMMAC |
31.55 |
33.58 |
2.03 |
| 11 |
Lozowski, Nicholas R (10)
|
AMSVSC |
33.57 |
33.67 |
0.10 |
| 12 |
Morgan, Tabari A (10)
|
AMRESY |
32.57 |
33.87 |
1.30 |
| 13 |
Punturiero, Salvatore A (10)
|
AMPEAQ |
37.38 |
34.88 |
-2.50 |
| 14 |
Pickett, Christopher B (8)
|
AMMAC |
34.40 |
41.08 |
6.68 |
|
Mitchell, Vincent P (10)
|
AMANSC |
33.73 |
NS |
- |
|
Yagusic, Jack A (10)
|
AMSASC |
34.35 |
NS |
- |