| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Behrend, Molly C (10)
|
MRWEST |
40.33 |
39.86 |
-0.47 |
| 2 |
DeCasseres, Brianna O (10)
|
MRWEST |
34.50 |
41.81 |
7.31 |
| 3 |
Galimi, Maeve O (10)
|
MRWEST |
42.57 |
43.10 |
0.53 |
| 4 |
McMahon, Reilly E (10)
|
MRWISC |
43.49 |
43.57 |
0.08 |
| 5 |
Lin, Rong Jia L (10)
|
MRQNS |
44.39 |
46.10 |
1.71 |
| 6 |
Weinstein, Sophie J (8)
|
MRWEST |
47.00 |
46.39 |
-0.61 |
| 7 |
Sherman, Alexa L (10)
|
MRLIE |
|
50.54 |
- |
| 8 |
Weinstein, Emma J (7)
|
MRWEST |
53.45 |
55.85 |
2.40 |
| 9 |
Dellagiovanna, Sofia M (10)
|
MRLIE |
|
58.80 |
- |
| 10 |
Galimi, Grace K (8)
|
MRWEST |
1:00.38 |
59.02 |
-1.36 |
| 11 |
Kohner, Paige A (7)
|
MRLIE |
|
1:01.20 |
- |
| 12 |
Cokley, Isabelle E (8)
|
MRLIE |
|
1:14.27 |
- |