| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Newsome, George W (10)
|
NIPMSC |
47.29 |
45.83 |
-1.46 |
| 2 |
Frenzel, Jack W (9)
|
NIWEBS |
51.39 |
49.04 |
-2.35 |
| 3 |
Richards-Dyson, Jax E (10)
|
NIGCOM |
47.55 |
50.19 |
2.64 |
| 4 |
Kendall, Logan F (10)
|
NIMERC |
45.41 |
50.35 |
4.94 |
| 5 |
Cottone, Cameron A (10)
|
NIGCOM |
1:01.59 |
54.55 |
-7.04 |
| 6 |
Potter, Logan C (10)
|
NIGRAT |
1:01.81 |
56.38 |
-5.43 |
| 7 |
Diebold, Matthew E (10)
|
NIGRAT |
|
56.97 |
- |
| 8 |
Ogletree, Blaine A (10)
|
NIFAST |
52.98 |
57.22 |
4.24 |
| 9 |
Spalty, Scott A (10)
|
NIGCOM |
1:23.20 |
1:01.22 |
-21.98 |
| 10 |
Coon, Parker R (10)
|
NIGCOM |
1:00.70 |
1:04.75 |
4.05 |
| 11 |
Pogroszewski, Noah Z (10)
|
NIGCOM |
1:00.28 |
1:09.30 |
9.02 |
|
Spalty, Dylan K (10)
|
NIGCOM |
58.54 |
NS |
- |
|
Zayas, Braden W (10)
|
NIGCOM |
54.00 |
NS |
- |