| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Scogna, Charlotte K (9)
|
PVFISH |
33.28 |
33.47 |
0.19 |
| 2 |
Dunn, Zoe E (9)
|
PVNCAP |
37.26 |
35.06 |
-2.20 |
| 3 |
Swanson, Esther M (9)
|
PVMACH |
40.18 |
36.35 |
-3.83 |
| 4 |
Ernst, Simone E (9)
|
PVYORK |
46.77 |
41.62 |
-5.15 |
| 5 |
Portella, Arianna M (9)
|
PVMACH |
51.47 |
41.96 |
-9.51 |
| 6 |
Spignardo, Calla A (9)
|
PVYORK |
|
45.02 |
- |
| 7 |
Cebrian, Francesca (9)
|
PVHACC |
1:04.10 |
47.05 |
-17.05 |
| 8 |
Gary, Morgan C (9)
|
PVNCAP |
|
47.42 |
- |
| 9 |
Nahid, Heidi (9)
|
PVNCAP |
50.85 |
47.94 |
-2.91 |
| 10 |
Williams, Maeve A (9)
|
PVMACH |
|
49.35 |
- |
| 11 |
Hayden, Sophie L (9)
|
VASTLH |
|
49.84 |
- |
| 12 |
Kowalczyk, Chloe H (9)
|
PVNCAP |
|
50.44 |
- |
| 13 |
Mikhin, Lydia (9)
|
PVMACH |
|
50.90 |
- |
| 14 |
Rhoads, Tara E (9)
|
PVYORK |
|
51.35 |
- |
| 15 |
Siraj, Hannah (9)
|
PVHACC |
|
52.14 |
- |
| 16 |
McGarry, Clare K (9)
|
PVYORK |
|
52.72 |
- |
|
Sayen, Madison G (9)
|
PVMACH |
|
DQ |
- |
|
Relay, Isabella M (9)
|
VASTLH |
|
DQ |
- |
|
Rojas, Elyse A (9)
|
VASTLH |
|
DQ |
- |
|
Pentala, Mona (9)
|
PVHACC |
|
DQ |
- |
|
Gleditsch, Caroline M (9)
|
PVNCAP |
|
NS |
- |
|
Vaughan, Alexis C (9)
|
PVMACH |
|
NS |
- |
|
Lechelt, Reese J (9)
|
VASTLH |
|
NS |
- |
|
Beckwith, Molly N (9)
|
PVYORK |
39.62 |
NS |
- |