| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Mahler, Olivia A (10)
|
MAHAC |
37.57 |
37.84 |
0.27 |
| 2 |
Shapiro, Madeline S (10)
|
MAUDAC |
38.81 |
38.02 |
-0.79 |
| 3 |
Foley, Elizabeth A (10)
|
MAHAC |
41.45 |
40.05 |
-1.40 |
| 4 |
Weiler-Timmins, Lillian S (10)
|
MAHAC |
44.44 |
42.12 |
-2.32 |
| 5 |
Sheldon, Jane A (10)
|
MAHAC |
42.81 |
43.88 |
1.07 |
| 6 |
Wang, Anna X (10)
|
MAHAC |
48.78 |
45.10 |
-3.68 |
| 7 |
Baughman, Caroline E (10)
|
MAUDAC |
46.38 |
45.38 |
-1.00 |
| 8 |
Roeting, Isabella P (10)
|
MAHAC |
47.02 |
46.38 |
-0.64 |
| 9 |
Lindsay, Grace E (10)
|
MAHAC |
52.00 |
53.29 |
1.29 |
|
Ardire, Anna C (10)
|
MAHAC |
52.25 |
DQ |
- |