| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Woronko, Julia M (10)
|
MAHAC |
35.01 |
34.68 |
-0.33 |
| 2 |
Moore, Lily A (10)
|
MAHAC |
37.82 |
39.23 |
1.41 |
| 3 |
Mahler, Olivia A (9)
|
MAHAC |
40.07 |
39.29 |
-0.78 |
| 4 |
Steckiel, Evangeline R (10)
|
MAGMAC |
40.57 |
41.00 |
0.43 |
| 5 |
Ely, Samantha L (9)
|
MAACWS |
44.64 |
41.84 |
-2.80 |
| 6 |
Huntsinger, Haley M (9)
|
MAGMAC |
|
44.96 |
- |
| 7 |
Weiler-Timmins, Lillian S (9)
|
MAHAC |
46.12 |
45.11 |
-1.01 |
| 8 |
Foley, Elizabeth A (9)
|
MAHAC |
45.08 |
45.44 |
0.36 |
| 9 |
Kerschner, Grace E (10)
|
MAGMAC |
43.33 |
46.48 |
3.15 |
| 10 |
Wyckoff, Emma L (9)
|
MAHAC |
49.46 |
47.77 |
-1.69 |
| 11 |
Forrester, Morgan W (10)
|
MAHAC |
50.47 |
49.02 |
-1.45 |
| 12 |
Verbos, Julieta M (10)
|
MAACWS |
48.34 |
49.79 |
1.45 |
| 13 |
Sheldon, Jane A (10)
|
MAHAC |
48.05 |
51.16 |
3.11 |
| 14 |
Czernecki, Anna G (9)
|
MAHAC |
50.66 |
51.80 |
1.14 |
| 15 |
Tewell, Jaden M (9)
|
MAACWS |
|
52.29 |
- |
| 16 |
Murray, Savannah J (9)
|
MAACWS |
53.97 |
52.54 |
-1.43 |
|
Wang, Anna X (9)
|
MAHAC |
|
DQ |
- |