| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Crisp, Caroline P (10)
|
NCWA |
35.83 |
33.26 |
-2.57 |
| 2 |
Riley, Lilly A (10)
|
NCGG |
33.42 |
33.70 |
0.28 |
| 3 |
Mungro-Turowski, Madison L (9)
|
NCMSC |
39.38 |
34.73 |
-4.65 |
| 4 |
Olguin-Abreu, Denise (10)
|
NCCVAC |
41.07 |
37.24 |
-3.83 |
| 5 |
Black, Rachel A (9)
|
NCRCST |
47.37 |
38.33 |
-9.04 |
| 6 |
Lynch, Kristen N (10)
|
NCGG |
41.60 |
38.45 |
-3.15 |
| 7 |
Rodriquez, Maria V (10)
|
NCMSC |
39.72 |
38.78 |
-0.94 |
| 8 |
Caudle, Riley S (10)
|
NCCVAC |
43.88 |
38.85 |
-5.03 |
| 9 |
Lopez, Marta B (9)
|
NCMSC |
44.87 |
38.88 |
-5.99 |
| 10 |
Lee, Jovie M (9)
|
NCCVAC |
42.82 |
39.01 |
-3.81 |
| 11 |
Negrete-Perez, Stephanie (10)
|
NCCVAC |
42.84 |
42.71 |
-0.13 |
| 12 |
Murphy, Riley J (10)
|
NCLENR |
39.31 |
43.06 |
3.75 |
| 13 |
Kale, Zelina M (10)
|
NCCVAC |
44.64 |
44.06 |
-0.58 |
| 14 |
Jones, Reilly E (9)
|
NCWA |
45.64 |
44.94 |
-0.70 |
| 15 |
LaBarge, Baylee D (10)
|
NCCVAC |
54.55 |
47.57 |
-6.98 |
| 16 |
Walters, Abigail R (10)
|
NCCVAC |
|
47.71 |
- |
| 17 |
Greene, Jordan A (9)
|
NCMSC |
|
48.05 |
- |
| 18 |
McNeal, Ella J (8)
|
NCGG |
|
48.09 |
- |
| 19 |
Van Meter, Claire V (9)
|
NCCVAC |
|
51.12 |
- |
| 20 |
Byrd, Legacy F (10)
|
NCCVAC |
57.81 |
51.19 |
-6.62 |
| 21 |
Cotter, Keira E (8)
|
NCWA |
|
52.16 |
- |
| 22 |
Gibson, Sailor E (9)
|
NCMSC |
|
58.79 |
- |
| 23 |
Braswell, Zoi (9)
|
NCLENR |
|
1:00.82 |
- |
| 24 |
Frye, Cailin M (9)
|
NCCVAC |
|
1:00.87 |
- |