| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Hedrick, Noah A (9)
|
NCCVAC |
35.08 |
33.54 |
-1.54 |
| 2 |
Carswell, Nathaniel R (10)
|
NCMSC |
34.18 |
33.62 |
-0.56 |
| 3 |
Peyton, William T (10)
|
NCGG |
36.82 |
35.10 |
-1.72 |
| 4 |
Olguin-Abreu, David F (10)
|
NCCVAC |
38.64 |
36.72 |
-1.92 |
| 5 |
Sexton, Brandon L (10)
|
NCMSC |
37.79 |
37.65 |
-0.14 |
| 6 |
Kines, Graham H (10)
|
NCGG |
|
38.34 |
- |
| 7 |
Dy, Logan X (10)
|
NCCVAC |
40.02 |
38.38 |
-1.64 |
| 8 |
Lunsford, Seth H (10)
|
NCGG |
|
39.52 |
- |
| 9 |
Stevens, Aedon C (10)
|
NCLENR |
43.18 |
40.17 |
-3.01 |
| 10 |
Maishman, Rowan W (9)
|
NCRCST |
45.30 |
43.21 |
-2.09 |
| 11 |
Hill, Caleb A (10)
|
NCRCST |
45.41 |
44.17 |
-1.24 |
| 12 |
Williams, Joseph R (9)
|
NCSAC |
|
44.29 |
- |
| 13 |
Cline, Jay C (9)
|
NCCVAC |
47.24 |
48.15 |
0.91 |
| 14 |
Blanco Garcia, Guillermo (10)
|
NCGG |
53.83 |
48.96 |
-4.87 |
| 15 |
Stroupe, Samuel S (9)
|
NCLENR |
|
49.51 |
- |
| 16 |
Kesterson, Christopher T (10)
|
NCRCST |
54.45 |
50.28 |
-4.17 |
| 17 |
Wilson, Connor A (10)
|
NCLENR |
1:05.26 |
1:10.02 |
4.76 |
|
Holland, Jacob F (10)
|
NCRCST |
|
DQ |
- |