| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Tabor, Beckett C (12)
|
NCGG |
30.98 |
30.92 |
-0.06 |
| 2 |
Peyton, William T (11)
|
NCGG |
32.03 |
31.27 |
-0.76 |
| 3 |
Carswell, Nathaniel R (11)
|
NCMSC |
33.44 |
33.32 |
-0.12 |
| 4 |
Major, Austin L (11)
|
NCGG |
34.12 |
33.44 |
-0.68 |
| 5 |
Sexton, Brandon L (11)
|
NCMSC |
37.65 |
34.02 |
-3.63 |
| 6 |
Torres, Jetiel J (12)
|
NCAQTC |
41.59 |
36.19 |
-5.40 |
| 7 |
Olguin-Abreu, David F (11)
|
NCCVAC |
36.72 |
38.66 |
1.94 |
| 8 |
Williams, Jaasiel L (12)
|
NCGG |
43.19 |
39.21 |
-3.98 |
| 9 |
Tracy, Peter C (11)
|
NCCVAC |
47.12 |
39.87 |
-7.25 |
| 10 |
Robinson, Jonah R (11)
|
NCGG |
43.18 |
41.81 |
-1.37 |
| 11 |
Moseley, Josiah L (11)
|
NCCVAC |
|
52.09 |
- |
| 12 |
Ramirez, Daniel A (11)
|
NCCVAC |
|
1:05.14 |
- |