| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Simmons, Roman S (9)
|
NCGCY |
43.69 |
44.91 |
1.22 |
| 2 |
Wagner, Dean T (10)
|
NCFAST |
44.68 |
45.04 |
0.36 |
| 3 |
Gaillard, Parker J (10)
|
NCTYDE |
46.95 |
47.06 |
0.11 |
| 4 |
Kelty, Maximillian C (10)
|
NCYSST |
48.37 |
48.06 |
-0.31 |
| 5 |
Kinsey, Kaden (9)
|
GAUNAT |
50.06 |
50.23 |
0.17 |
| 6 |
O'Donnell, Colin J (10)
|
NCTYDE |
50.06 |
50.29 |
0.23 |
| 7 |
Kafitz, Brandon J (9)
|
NCRACY |
58.07 |
55.57 |
-2.50 |
| 8 |
Hawkins III, Rodney D (10)
|
NCRACY |
51.72 |
58.02 |
6.30 |
| 9 |
McCrae, Bradley G (8)
|
SCRAYS |
53.59 |
58.35 |
4.76 |
| 10 |
Grant, Fischer K (7)
|
NCTYDE |
55.43 |
59.81 |
4.38 |