| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Leatherman, Brady H (15)
|
NCCVAC |
49.36 |
49.72 |
0.36 |
| 2 |
McRea, Micah W (15)
|
NCCVAC |
49.51 |
50.24 |
0.73 |
| 3 |
Morris, Nicholas K (16)
|
NCSAC |
50.90 |
52.30 |
1.40 |
| 4 |
Murphy, Samuel A (17)
|
NCLENR |
51.88 |
52.74 |
0.86 |
| 5 |
Burgess, Matthew D (16)
|
NCCVAC |
52.60 |
53.33 |
0.73 |
| 6 |
Little III, Thomas W (15)
|
NCCVAC |
54.08 |
54.46 |
0.38 |
| 7 |
Lari, Jackson W (17)
|
NCSAC |
54.71 |
55.09 |
0.38 |
| 8 |
Davison, Kenneth L (16)
|
NCGG |
56.22 |
56.05 |
-0.17 |
| 9 |
Negrete-Abreu, Isreal (15)
|
NCCVAC |
57.31 |
56.48 |
-0.83 |
| 10 |
Sacchetti, Mario T (15)
|
NCMSC |
59.70 |
57.53 |
-2.17 |
| 11 |
Striegel, Daniel L (16)
|
NCLENR |
1:00.58 |
57.60 |
-2.98 |
| 12 |
Vandergrift, Joshua (16)
|
NCWA |
1:00.50 |
58.30 |
-2.20 |
| 13 |
Walker, Jalon M (18)
|
NCLENR |
1:05.99 |
1:02.80 |
-3.19 |
| 14 |
Teague, Jeffery B (15)
|
NCWA |
1:00.86 |
1:03.09 |
2.23 |
| 15 |
Cooper, Christian B (17)
|
NCWA |
1:06.32 |
1:03.99 |
-2.33 |
| 16 |
Williams, Brandon L (16)
|
NCWA |
|
1:08.03 |
- |
| 17 |
Tabor, Beckett C (10)
|
NCGG |
1:17.36 |
1:16.26 |
-1.10 |
| 18 |
Major, Zachary A (12)
|
NCGG |
1:18.50 |
1:16.65 |
-1.85 |
|
Guerriero, Nicholas D (16)
|
NCSAC |
53.32 |
NS |
- |
|
Thompson, John M (17)
|
NCMSC |
56.91 |
NS |
- |