| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Miller, Brinley J (8)
|
NCNMA |
28.67 |
25.32 |
-3.35 |
| 2 |
Handy, Elsie R (8)
|
NCCVAC |
25.10 |
25.98 |
0.88 |
| 3 |
Ashcraft, McKenzie I (8)
|
NCNMA |
27.29 |
26.97 |
-0.32 |
| 4 |
Endsjo, Carolina I (8)
|
NCCVAC |
28.11 |
27.72 |
-0.39 |
| 5 |
Stancil, Ellie G (7)
|
NCGG |
31.33 |
29.16 |
-2.17 |
| 6 |
Vandergrift, Katelyn E (8)
|
NCWA |
31.15 |
29.43 |
-1.72 |
| 7 |
Millsaps, Katherine G (5)
|
NCCVAC |
38.60 |
34.75 |
-3.85 |
| 8 |
Bartlett, Veronica D (6)
|
NCCVAC |
38.55 |
40.30 |
1.75 |
| 9 |
Beltran-Trejo, Fernanda (6)
|
NCCVAC |
44.28 |
46.31 |
2.03 |
|
Cable, Meris A (7)
|
NCGG |
|
DQ |
- |
|
Bennett, Addison R (8)
|
NCRCST |
23.22 |
NS |
- |