| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Schneider, Taia M (9)
|
NCRCST |
39.42 |
37.46 |
-1.96 |
| 2 |
Mungro-Turowski, Madison L (10)
|
NCMSC |
39.01 |
37.70 |
-1.31 |
| 3 |
Tysinger, Alexandra N (10)
|
NCSAC |
|
40.57 |
- |
| 4 |
Lopez, Marta B (10)
|
NCMSC |
42.18 |
41.42 |
-0.76 |
| 5 |
Lopez-Rodriquez, Neyli M (9)
|
NCMSC |
44.69 |
42.88 |
-1.81 |
| 6 |
Kohler, Madison N (10)
|
NCWA |
43.98 |
43.43 |
-0.55 |
| 7 |
Black, Rachel A (10)
|
NCRCST |
40.90 |
43.63 |
2.73 |
| 8 |
Hollar, Caylee A (9)
|
NCCVAC |
46.29 |
44.92 |
-1.37 |
| 9 |
Schiffel, Karsen L (9)
|
NCCVAC |
59.70 |
57.29 |
-2.41 |
| 10 |
Hill, Addison C (9)
|
NCRCST |
1:13.78 |
1:06.89 |
-6.89 |
| 11 |
Baynard, Zoie L (10)
|
NCSAC |
|
1:10.64 |
- |
|
Thornburg, Ardyn L (10)
|
NCGG |
42.01 |
DQ |
- |