| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Smitherman, Katherine M (10)
|
NCTYDE |
40.91 |
40.59 |
-0.32 |
| 2 |
White, Riley E (10)
|
SCYCSC |
42.25 |
43.00 |
0.75 |
| 3 |
Johnson, Valerie D (9)
|
NCYSST |
50.67 |
47.19 |
-3.48 |
| 4 |
Christian, Abigail C (9)
|
SCYCSC |
48.37 |
48.86 |
0.49 |
| 5 |
Shellem, Addison P (8)
|
SCYCSC |
51.71 |
49.27 |
-2.44 |
| 6 |
Cinson, Evelyn S (9)
|
NCYSST |
54.46 |
53.82 |
-0.64 |
| 7 |
Henderson, Mya B (9)
|
SCNYST |
55.88 |
55.55 |
-0.33 |
| 8 |
Hilton, McKinley B (10)
|
NCYSST |
1:04.55 |
56.64 |
-7.91 |
| 9 |
Bush, Dakota L (10)
|
SCNYST |
1:01.03 |
57.59 |
-3.44 |
| 10 |
Bartlett, Veronica D (10)
|
NCYSST |
1:03.79 |
59.70 |
-4.09 |
| 11 |
Luhm, Evie J (10)
|
NCWNCY |
1:10.44 |
1:00.37 |
-10.07 |
| 12 |
Orellana, Mia (9)
|
SCSYFF |
1:15.04 |
1:00.79 |
-14.25 |
| 13 |
Burgess, Emily C (10)
|
SCNYST |
1:08.01 |
1:03.91 |
-4.10 |
| 14 |
Sechriest, Emory G (10)
|
NCRACY |
1:04.90 |
1:05.16 |
0.26 |
| 15 |
Johnson, Erika G (7)
|
NCYSST |
1:05.67 |
1:07.04 |
1.37 |
| 16 |
Maradei, Addison M (9)
|
SCYCSC |
1:13.44 |
1:10.97 |
-2.47 |
| 17 |
Moore, Allaynah A (8)
|
SCNYST |
2:07.94 |
1:17.62 |
-50.32 |
| 18 |
Shellem, Aubrey P (7)
|
SCYCSC |
1:29.98 |
1:28.90 |
-1.08 |
|
Erby, Sloane A (8)
|
NCYSST |
1:24.67 |
DQ |
- |
|
Ward, Maddison L (9)
|
NCYBAC |
1:12.17 |
DQ |
- |
|
Millsaps, Anna Claire C (5)
|
NCYSST |
1:28.67 |
DQ |
- |
|
Millsaps, Caroline M (7)
|
NCYSST |
1:08.67 |
DQ |
- |
|
Sprinkle, Ella G (10)
|
NCYSST |
1:10.67 |
DQ |
- |