| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Hitchcock, Ella B (11)
|
NCYSST |
33.12 |
32.51 |
-0.61 |
| 2 |
Towne, Mia N (12)
|
NCWNCY |
38.89 |
33.74 |
-5.15 |
| 3 |
Goodman, Merrit S (12)
|
NCMAC |
31.47 |
33.77 |
2.30 |
| 4 |
Kjar, Ava M (12)
|
NCWNCY |
38.84 |
34.61 |
-4.23 |
| 5 |
Kondal, Meera A (11)
|
NCWNCY |
34.60 |
35.47 |
0.87 |
| 6 |
Wiley, Cacey A (11)
|
NCMAC |
38.89 |
36.42 |
-2.47 |
| 7 |
Ray, Mallory E (12)
|
NCWNCY |
|
37.55 |
- |
| 8 |
Shields, Shaelyn G (11)
|
NCMAC |
|
37.85 |
- |
| 9 |
Strouse, Sullivan R (11)
|
NCMAC |
43.14 |
42.07 |
-1.07 |
| 10 |
Eldreth, Madison G (11)
|
NCUN |
|
42.46 |
- |
| 11 |
Krupinski, Harlow M (11)
|
NCMAC |
|
50.13 |
- |
| 12 |
Bailey, Payton C (11)
|
NCMAC |
|
52.97 |
- |
|
Chambers, Savannah M (11)
|
NCYSST |
|
DQ |
- |
|
Darwish, Danielle E (11)
|
NCMAC |
|
NS |
- |
|
Price, Nora A (11)
|
NCWNCY |
|
NS |
- |