| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Lineberger, Gracie B (16)
|
NCRACY |
27.44 |
26.81 |
-0.63 |
| 1 |
Waters, Zoey L (15)
|
NCRACY |
27.51 |
27.38 |
-0.13 |
| 1 |
Sechriest, Addyson M (15)
|
NCRACY |
27.63 |
27.38 |
-0.25 |
| 1 |
Mangum, Kaylee A (15)
|
NCNCAC |
30.68 |
29.66 |
-1.02 |
| 1 |
Leas, Reagan E (16)
|
NCVACB |
34.37 |
34.78 |
0.41 |
| 2 |
McCrimmon, Quinn A (17)
|
NCHAC |
27.05 |
26.95 |
-0.10 |
| 2 |
Brown, Sachi (15)
|
NCYBAC |
31.98 |
32.57 |
0.59 |
| 3 |
Su, Alicia (16)
|
NCNCAC |
27.38 |
27.42 |
0.04 |
| 3 |
Merlette, Elizabeth A (15)
|
NCYBAC |
28.37 |
27.83 |
-0.54 |
| 3 |
Bacigalupo, Lillian G (17)
|
NCYBAC |
30.53 |
32.77 |
2.24 |
| 4 |
Gallo, Helen (15)
|
NCNCAC |
28.00 |
28.06 |
0.06 |
| 4 |
Kilgallen, Kathryn E (17)
|
NCNCAC |
27.44 |
29.46 |
2.02 |
| 5 |
Maiden, Reagan (16)
|
NCRACY |
27.89 |
29.03 |
1.14 |
| 6 |
Sechriest, Makenzie R (17)
|
NCRACY |
28.47 |
29.23 |
0.76 |
| 7 |
Zagol, Charlotte J (15)
|
VAHAVA |
29.68 |
29.55 |
-0.13 |
| 8 |
Li, Joie N (15)
|
NCNCAC |
29.72 |
30.96 |
1.24 |
| 9 |
Giovanello, Aurora J (16)
|
NCNCAC |
28.83 |
31.21 |
2.38 |
|
Mijangos, Iran Y (16)
|
NCVACB |
36.23 |
36.72 |
0.49 |
|
Folger, Kathryn C (16)
|
NCVACB |
30.84 |
NS |
- |
|
Brown, Jade T (15)
|
NCNCAC |
30.22 |
NS |
- |