| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Rypkema, Ava R (12)
|
NCGFSC |
34.59 |
33.99 |
-0.60 |
| 1 |
Wesley, Anna C (11)
|
NCACCH |
40.20 |
36.85 |
-3.35 |
| 1 |
Girouard, Madelyn G (12)
|
NCRMY |
38.07 |
36.86 |
-1.21 |
| 2 |
Manners, Ava J (11)
|
NCGFSC |
34.66 |
36.92 |
2.26 |
| 2 |
Brewer, Rylee G (11)
|
NCRMY |
37.79 |
37.29 |
-0.50 |
| 2 |
Mondul, Angelia R (11)
|
VADYST |
40.64 |
41.22 |
0.58 |
| 3 |
Wrenn, Emily L (11)
|
NCGFSC |
35.36 |
37.49 |
2.13 |
| 3 |
Turner, Jillian B (12)
|
NCGFSC |
40.12 |
41.42 |
1.30 |
| 4 |
Tanner, Olivia M (11)
|
NCACCH |
45.00 |
42.02 |
-2.98 |
| 5 |
Lineburger, Gracie B (12)
|
NCRACY |
42.68 |
42.50 |
-0.18 |
| 6 |
Goodnight, Caroline R (11)
|
NCACCH |
50.11 |
45.43 |
-4.68 |
| 7 |
Pope, Betsy B (11)
|
NCACCH |
45.18 |
45.67 |
0.49 |
| 8 |
Jernigan, Jordyn M (11)
|
NCRACY |
46.78 |
46.59 |
-0.19 |